Extraction and Identification of Chemical Composition within Acetone Extract of Taxus baccata L. Bark

Afraz, Seddigheh; Hosseinihashemi, Seyyed Khalil

doi:10.22092/ijwpr.2017.115389.1436

Document Type : Research Paper

Authors

¹ M.Sc., Department of Wood Science and Paper Technology, Karaj Branch, Islamic Azad University, Karaj, Iran

² Associate Prof., Department of Wood Science and Paper Technology, Karaj Branch, Islamic Azad University, Karaj, Iran

https://doi.org/10.22092/ijwpr.2017.115389.1436

Abstract

In this research, the bark extract of the yew (Taxus baccata L.) tree trunk grow in AfraTakhte forest cache of Aliabad-e Katul) located in Golestan province was extracted with acetone solvent and by Soxhlet apparatus method and then the chemical components within it were identified. The bark of prepared disks was separated and converted to the flour. Afterwards, extract insoluble acetone solvent was prepared according to TAPPI standard method. In order to silylation of compounds, amount of dry powder of extract was poured into a glass vial and the derivative materials (pyridine, BSTFA + TMCS 1%) were added to it and to identification the sample was injected to the gas chromatography- mass spectrometry device. The results from this research indicated that the mean value of acetone extract of yew bark was 13.63%. Generally, 46 compounds were identified in the yew bark. The most abundant identified compounds were including: tetrasiloxane, decamethyl- (14.80%), hexadecanoic acid, hydroxy ester (11.24%), 3-(3-aminopropyl)-6-(3,3-dimethylallylamino)purine (9.85%), d-ribofuranose, 1,2,3,5-tetrakis-O-(hydroxy)- (9.31%), 2-methylpyrrolo[1,2-a]pyrazin-1(2H)-one (6.73%). The various chemical components such as phenolic, flavenoid, alkaloid, fatty acid, fatty acid ester, sugar, alkane, fatty alcohol, alcoholic sugar, and stroied were identified in the acetone extract of yew bark that some of them can synthesized as preservatives for inhibition of wood-degrading organisms. The most important identified cytotoxic and anticancer compounds in the acetone extract of yew bark has been 4-piperidinone, 2,2,6,6-tetramethyl- (0.19%) and phenol, 2,4-bis(1,1-dimethylethy)- (3.32%).

Keywords

Main Subjects

Chemistry of wood

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Iranian Journal of Wood and Paper Science Research

Extraction and Identification of Chemical Composition within Acetone Extract of Taxus baccata L. Bark

References

References

Volume 33, Issue 1 - Serial Number 62
April 2018
Pages 88-99

Extraction and Identification of Chemical Composition within Acetone Extract of Taxus baccata L. Bark

References

References

Volume 33, Issue 1 - Serial Number 62April 2018Pages 88-99

Volume 33, Issue 1 - Serial Number 62
April 2018
Pages 88-99