Investigation on the influence of nutrients and treatment period on enzyme activities of Phanerochaete chrysosporium

Sharari, Meraj; Jahan Latibari, Ahmad; Rafiei, Gholamreza; Mirshokraei, Ahmad

doi:10.22092/ijwpr.2010.117314

Document Type : Research Paper

Authors

¹ 1- Ph.D. Candidate, College of Natural Resources, University of Tehran, Karaj, Iran

² 2- Associate Prof., Agriculture Research Center Islamic Azad University, Karaj Branch, Karaj, Iran

³ Associate Prof. College on Natural Resources, University of Tehran, Karaj, Iran

⁴ Professor, Payam-e-Nor University, Tehran, Iran

https://doi.org/10.22092/ijwpr.2010.117314

Abstract

In the course of enhanced bioremediation, addition of nutrients such as carbon, nitrogen, phosphorous, etc, substrates, electron acceptors as well as vitamins (Thiamin, etc) and veratryl alcohol, increased metabolic activities, will influence the production of enzymes and enhances the deterioration of pollutants. In this study, the effect of 1% (V/V) of nutrients containing various mineral salts, carbon (glucose and malt), nitrogen (casein peptone yeast extract) and some enzyme inducer, such as veratryl alcohol and thiamin hydrochloride on production of laccase, peroxidase and xylanase enzymes by Phanerochaete chrysosporium in bagasse preparation effluent is investigated. The results showed that MSB+MCTV is the most effective additive which causes the highest lignolitic activity of this fungus after 5 to 7 days in case of laccase and peroxidase and 3 days for xylanase, and maximizes the production of the enzymes. The production of these enzymes is measured at 78, 193 and 60 U/l respectively. However, in conditions without additives, the relevant values are 26, 54 and 16 U/l respectively. The relation between different stages of fungus growth and enzyme production and changes in lignin and carbohydrate of effluent after the period of nine days is also investigated.

Keywords

Main Subjects

Biology and Wood Protection

References

- Abdel-Satera, M. A. & El-Said, A. H. M., 2001, Xylan-decomposing fungi and xylanolytic activity in agricultural and industrial wastes, International Biodeterioration & Biodegradation, 47: 15-21.

- Anjaneyulu, Y., Chary, N. S. & Raj, D. S. S., 2005, Decolourization of Industrial Effluents – Available Methods and Emerging Technologies– A Review, Environmental Science and Biotechnology, 4 (4).

- Asamudo, N. U., Daba, A. S. & Ezeronye, O. U., 2005, Bioremediation of textile effluent using Phanerochaete chrysosporium, African Journal of Biotechnology, 4 (13): 1548-1553.

- Bocchini, D. A. , Oliveira, O. M. M. F, Gomes, E. F., & Silva, R. D., 2005, Use of sugarcane bagasse and grass hydrolysates as carbon sources for xylanase production by Bacillus circulans in submerged fermentation, Process Biochemistry, 40: 3653–3659.

- Chance, B. & Maehly, A. C., 1955, Method in Enzymology II, 773-775

- Chen, W. P., Matsuo, M., & Yasui, T., 1986, Purification and characterization of a neutral endoxylanase from Aspergillus nidulans, Agriculture Biology Chemistry, 50: 118

- Cho, N.S., Kim, D. H., Cho, H. Y., Ohga, S. & Leonowicz, A., 2006, Effect of Various Compounds on the Activity of Laccases from Basidiomycetes and Their Oxidative and Demethoxylating Activities J. Fac. Agr., Kyushu Univ, 51 (2): 211–218 .

- Colombo, R., Lanc¸ F., Yariwake, M. J. H., 2006, Determination of flavonoids in cultivated sugarcane leaves, bagasse, juice and in transgenic sugarcane by liquid chromatography-UV detection, Journal of Chromatography, 1103: 118–124.

- Dahiya, J., Singh, D. & Nigam, P., 2001, Decolourization of synthetic and spentwash melanoidins using the white- rot fungus Phanerochaete chrysosporium JAG-40, Bioresource Technology, 78 (1): 95-98.

- Dosoretz, C. G., Dass, S. B., Reddy, C. A.& Grethlein, H. E., 1990, Protease-Mediated Degradation of Lignin Peroxidase in Liquid Cultures of Phanerochaete chrysosporium ,Applied and environmental microbiology, 56 (11): 3429-3434.

- Driessel, B. V., 2003, Bioremediation of a bleach plant effluent from the pulp and paper industry, In the Department of Microbial, Biochemical and Food Biotechnology, Faculty of Natural and Agricultural Sciences, University of the Free State, Bloemfontein.

- Jeffries, T. W., Choi, S. & Kent K. T., 1981, Nutritional Regulation of Lignin Degradation by Phanerochaete chrysosporium, Applied and environmental microbiology, 42 (2): 290-296.

- Kanaly, R. A., Hur, H., 2006, Growth of Phanerochaete chrysosporium on diesel fuel hydrocarbons at neutral pH, Chemosphere, 63: 202–211

- Kumar, V., Wati, L., Nigam, P., Banat, I. M., Yadav, B. S., Singh D. & Marchan, R., 1998, Decolorization and biodegradation of anaerobically digested sugarcane molasses spent wash effluent from biomethanation plants by white-rot fungi, Process Biochemistry, 33 (1): 83-88.

- Kunz, A., Reginatto, V., Duran, N., 2001, Combined treatment of textile effluent using the sequence Phanerochaete chrysosporium – ozone, Chemosphere, 44: 281-287.

- Lacina, C., Germain, G., Spiros, A. N., 2003,Utilization of fungi for biotreatment of raw wastewaters, African Journal of Biotechnology, 2 (12): 620-630 .

- Meysami, P., 2001, Feasibility Study of Fungal Bioremediation of a Flare Pit Soil Using White Rot Fungi, Department of chemical and petroleum engineering, The university Calgary, Alberta.

- official test method, 1983, Acid soluble and insoluble lignin in wood and pulp, TAPPI, T 222 om-83.

- Perez, J., Mun, J. Dorado, O., Rubia, T. & Martnez, J., 2002, Biodegradation and biological treatments of cellulose, hemicellulose and lignin: an overview, Int Microbiol 5: 53–63.

- Radha, K. L., Regupathi, I., Arunagiri, A., Murugesan, T., 2005, Decolorization studies of synthetic dyes using Phanerochaete chrysosporium and their kinetics; process Biochemistry, 40: 3337 – 3345.

- Ride, J.P., 1980, Physiological Plant pathology, 16: 187- 196.

- Sheldon, M.S., Mohammed, K., Ntwampe, S. K. O., 2007, An investigation of biphasic growth kinetics for Phanerochaete chrysosporium (BKMF-1767) immobilized in a membrane gradostat reactor using flow-cells, Enzyme and Microbial Technology .

-Shirish., 2007, Bioremediation of pulp and paper mill effluent by Phanerochaete chrysosporium, Shirish’s Environmental Portal.

- Travis, G. R., 2007, An overview of sugar culture in Morocco, particularly within a Berber community in Rastabouda, School of Biological Sciences ,University of Canterbury.

-Turpeinen, B. K., 2007, Lignocellulose degradation and humus modification by the fungus Paecilomyces inflatus, Division of Microbiology Department of Applied Chemistry and Microbiology, University of Helsinki.

- Vicuna, R., 2000, Ligninolysis: A very peculiar microbial process. Molecular Biotechnology, 14: 173 -176.

- What Is Bioremediation, 2005, Transgalactic Ltd, manufacturer of Bioscreen C software.

- Wu, J., Xiao, Y. & Yu, H., 2005, Degradation of lignin in pulp mill wastewaters by white-rot fungi on biofilm, Bioresource Technology, 96 (12): 1357-1363 .

Iranian Journal of Wood and Paper Science Research

Investigation on the influence of nutrients and treatment period on enzyme activities of Phanerochaete chrysosporium

References

References

-Shirish., 2007, Bioremediation of pulp and paper mill effluent by Phanerochaete chrysosporium, Shirish’s Environmental Portal.

Volume 25, Issue 2 - Serial Number 33
September 2018
Pages 275-290

Investigation on the influence of nutrients and treatment period on enzyme activities of Phanerochaete chrysosporium

References

References

-Shirish., 2007, Bioremediation of pulp and paper mill effluent by Phanerochaete chrysosporium, Shirish’s Environmental Portal.

Volume 25, Issue 2 - Serial Number 33September 2018Pages 275-290

Volume 25, Issue 2 - Serial Number 33
September 2018
Pages 275-290