Document Type : Research Paper

Authors

1 Professor, Dept. of Chemistry, Payame Noor University,Ozgol, Tehran, Iran

2 Ph.D student,Dept. of wood and Paper, Faculty of Natural Resources,University of Tehran, Karaj, Iran

3 Associate Professor, Dept. of wood and Paper, Faculty of Natural Resources, University of Tehran ,Karaj, Iran

Abstract

     In this paper, a novel enzymatic method was evaluated for isolation and purification of wood lignin. The chemical structure of enzymatic lignin (Cellulytic lignin) was compared with conventional Bjorkman method (Milled wood lignin). The chemical structure elucidation was performed with FTIR, quantative 13CNMR, basic elemental analysis and Gel Permeation Chromatography (GPC). The results showed that enzymatic lignin preparation has a better chemical structure than MWL. Comparing to conventional isolation methods, application of an enzyme sequence with reduced milling time could lead to a mild extraction with less structural degradation of lignin. Resulting lignin could be more representative for wood lignin than milled wood lignin.

Keywords

Main Subjects

Adler, E. and S. Hernestam (1955) Estimation of phenolic hydroxyl groups in lignin. 1. Periodate oxidation of guaiacol compounds. Acta Chem. Scand., 9, 319-334.
Bjorkman A. (1954) Isolation Lignin from Finly Divided Wood with Natural Solvent, Nature 174:1057-1058
Blakeney A.B., P.J. Harris,R.J. Henry, B.A. Stone (1983) A simple and rapid preparation of alditol acetates for monosaccharide analysis. Carbohydr. Res. 113:291–299.
Capanema E. A., M. Y. Balakshin and  J. F. Kadla (2005) Quantitative Characterization of a Hardwood Milled Wood Lignin by Nuclear Magnetic Resonance Spectroscopy, J. Agric. Food Chem. 53, 96399649
Chang H. M., Cowling E. B., Brown W., Adler E., Miksche G. (1975) Comparative Studies on Cellulolytic Enzyme Lignin and Milled Wood Lignin of Sweetgum and Spruce. Holzforschung, 29, 153-159.
Duarte A. (2001) Eucalyptus globules kraft pulping residual lignin, Holzforschung, 55, 645-651.
Esterbauer H, W. Steiner, I. Labudova (1991). Production of Trichoderma cellulase in laboratory and pilot scale. Biores. Technol.36, 51-6.
Faix O. (1992) Fourier transforms infrared spectroscopy. In: Lin SY, Dence CW, editors. Methods in lignin chemistry. Berlin: Springer-Verlag; . p. 83–109.
Faix O., Beinhoff O. (1988) FTIR spectra of miled wood lignins and lignin polymer models (DHPs) with enhanced resolution obtained by deconvolution, J. Wood Chem. Technol., 8, 505-522.
Fujimoto A., Y. Matsumoto, H.M. Chang, G. Meshitsuka (2005) Quantitative evaluation of milling effects on lignin structure during the isolation process of milled wood lignin. J. Wood Sci. 51:89–91.
Furuno H., T. Takano, S. Hirosawa, H. Kamitakahara and F. Nakatsubo (2006) Chemical structure elucidation of total lignins in Woods. Part II: Analysis of a fraction of residual wood left after MWL isolation and solubilized in lithium chloride/N,N-dimethylacetamide Holzforschung, Vol. 60, pp. 653–658
Gellerstedt, G.; Northey, R. A.  (1989) Analysis of Birch Wood Lignin by Oxidative-Degradation. Wood Sci. Technol.  23, 75-83.
Goyal A, B. Ghosh, D. Eveleigh (1991) Characterisation of Funga Cellulases. Biores. Technol. 36:37-50.
Higuchi T. (1985) Biosynthesis and Biodegradation of Wood Components; Academic Press: Orlando, FL.
Holtman K.M., H.M.  Chang  and  J.F. Kadla (2004) Solution-State Nuclear Magnetic Resonance Study of the Similarities between Milled Wood Lignin and Cellulolytic Enzyme Lignin, J. Agric. Food Chem. 52: 720-726
Hon, D. N. S.; Glasser, W. G. (1979) Effect of Mechanical Action on Wood and Fiber Components. Tappi, 62, 107-110.
Hu Z., T.F. Yeh, H.M. Chang,Y. Matsumoto  and J.F. Kadla (2006) Elucidation of the structure of cellulolytic enzyme lignin, Holzforschung, 60, 389–397
Ibarra D., J. C. del R´ıo, A. Gutiérrez, I. M. Rodr´ıguez, J. Romeroc, M. J. Martınez, Á.T. Martınez  (2004) Isolation of high-purity residual lignins from eucalypt paper pulps by cellulase and proteinase treatments followed by solvent extraction, Enzyme and Microbial Technology 35: 173–181
Ikeda T.,K. Holtman, J.F. Kadla,H.M.  Chang,H.  Jameel (2002) Studies on the effect of ball milling on lignin structure using a modified DFRC method. J. Agric. Food Chem. 50:129–