Document Type : Research Paper
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Abstract
Abstract:
Background and aim: As a result of population growth, increase in paper and wood products consumption, and restrictions on using raw materials, producing ligni-culture with black poplar and poplar as fast-growing spices have found their own special status and proponents. The aim of this study was accomplished on identification and comparison of the phenolic chemical components in wood of black poplar (populous nigra) and poplar (populous deltoides) tree.
Materials and methods: In this study, three trees from species of planted black poplar and poplar tree were randomly cut down in planted forest region of Nowshar city. Then from each tree were separated three disks, at first wood flour and then extractive measured by TAPPI standards. The wood flour was washed by acetone and extractives residue was added BSTFA reactor. In order to identification of extract, about 1mg solid extracts obtained, mixed with 30 micro liter BSTFA reagent and about 15 micro liter pyridine inside tube test. The samples kept in Ben Marry Bath in 70 oC for an hour and they were analyzed by GC/MS. Then the samples analyzed by using GC/MS on an HP 6890 Gas Chromatograph, equipped with a split/split less injector and a 5973 Mass Selective Detector (MSD). The column oven was programmed as follows: Chromatography was performed on a HP-5MS capillary column (SGE, 30 m, 0.25 mm), kind of carrier gas, Helium with 1ml/min speed and temperature program between 60 – 260 °C, increase temperature 6 °C/min. For the identification of compounds were used GC diagram which shows abundance and retention time of each compound, calculation of quartz index and Adams table. The retention indices were calculated for all volatile constituents using a homologous series of C9 to C19 n-alkanes. For the Identification of compounds were used GC diagram which shows abundance and retention time of each compound, and calculation of quartz index and Adams table. The retention indices were calculated for all volatile constituents using a homologous series of C9 to C19 n-alkanes.
I=100n+100(trx-trn)/(trn+1-trn)
Where: I: quartz index, n: Carbon number of normal alcan, trn+1: Retention time of unknown compound, trn: Retention time of normal alcan.
Results: The result of GC-MS showed that there were 68 and 105 compounds in wood of black poplar and poplar tree, respectively. That di-Limonene (30.26%), bis (2-ethylhexyl) phthalate (10.07%), Gamma-Sitosterol (1.06%), Hexadecanoic acid (2.0 %), gamma-Terpinene (4.02%), beta-Myrcene (0.58%), 5-methyl-3-Heptanol (1.35%) and 4-Hydroxy-Benzoic acid (3.62%) were the most important compounds in wood of poplar tree. There were the Limonene (25.62%), Anymol (5.29%), gamma-Terpinene (3.38%), 1,2-Benzandicarboxylic acid(2.00%), Benzen methanol (0.94%), Agarospirol(0.91%), Tetradecanoic acid(0.19%) and bis (2-ethylhexyl) phthalate (0.68%) as the most important compounds in wood of black poplar tree.
Conclusion: So that, the gas chromatographs showed that Limonene, Benzene methanol, alpha-Terpineol, beta-Bisabolene, Hexadecanoic acid and bis (2-ethylhexyl) phthalate were the 7 common compounds in the wood of black poplar and poplar tree, too. These compounds can play an important role in the durability and consumption of these woods.
Keywords: Black Poplar, Poplar, Retention time, BSTFA, Gas Chromatography, Mass Spectrometry.
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