Document Type : Research Paper

Authors

1 M.Sc., Wood and Paper Science and Technology, Dept. Karaj Branch, Islamic Azad University, Karaj, Iran

2 Professor, Wood and Paper Science and Technology, Dept. Karaj Branch, Islamic Azad University, Karaj, Iran

3 Associate Prof., Wood and Paper Science and Technology, Dept. Karaj Branch, Islamic Azad University, Karaj, Iran

Abstract

 Background and Objectives: Plant extractives show unique properties. They impart colors and change the color of wood and generates attractive appearance. Since early days, societies have been utilizing plant extractives in industrial, medical and even as fungicides and biocides. In early times this application was based on the professional experiences, but as the sciences were advanced, then the technical and experimental identification and utilization was employed. In general, people look at walnut tree wood and fruit as its economic values. However, scientific research has proved the values of walnut tree extractives. In this study the chemical composition of walnut tree extractives and its variation in two periods of spring and summer as well as the impact of the drying on quantity and quality of the extractive components are investigated.
Methodology: Walnut tree leaves from a selected location of an old walnut tree (100 years old) in mountain region of Karaj were collected in spring and summer. One part of the samples was air dried and another portion was used at green moisture condition. Totally four samples were obtained. Ethanol-acetone extraction was according to Tappi T204-05 procedure. The extractive was identified using GC-MASS spectroscopy and the chemical components in the extractives were identified and tabulated.
 Results: The results revealed that the dried leaves contain fewer chemical compounds compared to green samples which can be an indication that some of the compounds in green sample are volatile and vaporize upon drying. Furthermore, the extractives chemical composition and content varies between spring and summer samples. The major chemical compounds identified were 2-methylneptaline, tri-decan, tetradecan, 2,3-dimethyl naptaline and methylcyclobenzen.
Conclusion: The results indicated the seasonal variation of walnut tree extractives. These is also considerable variation in wet and dry samples which can be attributed to the presence of volatile compounds. The odor of walnut leaves is the consequence of existence of such volatile extractives. The absence of some of the compounds in summer samples indicate the seasonal changes of the extractives.

Keywords

Main Subjects

-Amaral, J.S., Seabra, R.M., Andrade, P.B., Valentao, P., Pereira, J.A. and Ferreres, F., 2004. Phenolic profile in the quality control of walnut (Juglans regia L.) leaves. Journal of Food Chemistry, 88:373-379.
-Forino, M., Stiuso, P., Lama, S., Ciminiello, P., Tenore, G.C., Noellino, E. and Taglialatela-Scafati, O. 2016. Bioassay- Guided identification of the antihyperglycaemic constituennts of walnut. Journal of Functional Foods. 26; 731-738.
-Gradt, I., Kuhn, S., Morsel, J.T. and Zvaigzne, G., 2017. Chemical composition of sea buckthorn leaves, branches and back. Proceeding of the Latvian academy of sciences. Section B, 71:211-216. 
-Hosseini Hashemi, S. Kh. and Jahan Latibari, A., 2011. Evaluation and identification of walnut heartwood extractives for protection of poplar wood. Bio Resources. 6(1); 59-69.
-Izadiyan, Z., Shameli, K., Miyake, M., Hara, H., Eva Binti Mohamad, S., Kalantari, K., Husnaa Mohd Tiab, S. and Rasouli, E. 2020. Cytotoxicity assay of plant-mediated synthesized iron oxide nanoparticles using Juglans regia green husk extract. Arabian Journal of Chemistry. 13(1); 2011-2023.
-Jabli, M., Sebeia, N., Baulares, M. and Faidi, K., 2017. Antibacterial potential, gas chromatography–mass spectroscopy and a full investigation of their dyeing properties. Journal of Industrial Crops and products. 108; 690-699.
-Oliveira, I., Sousa, A., Ferreira, I., Bento, A., Estevinho, L. and Pereira, J.A., 2008. Total phenols, antioxidant potential and antimicrobial activity of walnut (Juglans regia L.) green husks. Food and chemical Toxicology. 46; 2326-2331.
-Rezaei Arami, S., 2011. Phenolic compounds extraction of two walnut varieties leaves and green bark using micro waves and investigation the antioxidant and anti-microbial activities of the compounds. Food Industries Research, Vol. 22, no.1:41-50.
-Santos, A., Barros, L., Calhelha, R.C. and Duenas, M., 2013. Leaves and decoction of Juglans regia L.: Different performances regarding bioactive compounds and in vitro antioxidant and antitumor effects. Industrial Crops and Products. 51; 430-436.
-Sharafetichaleshtre, A., Sharafetichaleshtre, R., Sharafetichaleshtre, F. and Rafiean, M., 2010. Investigating the anti-bacterial effect of ethanol extract of walnut tree leaves on Peripeoni Bactereum Akne. Research J. of Zanjan Medical Sciences University, vol. 16, no. 71:42-49 (In Persian).
-Slatnar, A., Mikulic-petkovsek, M., Stamper, F., Veberic, R. and Solar, A., 2015. Identification and Quantification of phenolic compounds in kernels, oil and bagasse pellets of common walnut (Juglans regia L.). A journal of the Canadian Institute of Food Science and Technology (CIFST).67; 255-263.
-Solar,A., Colaric, M., Usenik,V. and Stamper, F., 2006. Seasonal variations of selected flavonoids, phenolic acids and quinones in annual shoots of common walnut (Juglans regia L.). Journal of Plant Science. 170; 453-461 (In Persian).
-Technical Association of Pulp and Paper Industry, 2020. Tappi test methods T204-05. Tappi Press, Atlanta Georgia, United States of America.
-Vaysi, R. and Habib P., 2022. Identification and comparison of organic chemical components in wood and bark of hornbeam and ironwood by GC-MS methods. Iranian Journal of Wood and Paper Research Vol. 37, no. 2:150-163. (In Persian).
-Vaysi, R., Malekian, B. and Masoudifar, M.,  2020. Identification and comparison of metallic ions and organic chemical components in wood and bark of mulberry tree by atomic absorption and GC-MS methods. Iranian Journal of Wood and Paper Research Vol. 35, no. 3:283-293 (In Persian).